FastQCFastQC Report
Fri 10 Feb 2017
ERR1148033.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148033.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7546103
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161220.21364669949509038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97850.12966957911918242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75730.10035643563306783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA180500.048.9445271
GGTATCA91700.045.8334771
AACGCAG206700.041.89786
TCAACGC207150.041.7206424
CAACGCA209900.041.2307135
ATCAACG216500.040.0287443
TATCAAC238850.036.4833072
ACGCAGA242300.035.791057
CGCAGAG248300.034.9022258
GCAGAGT285700.030.4582129
GTACATG266850.028.533221
ACATGGG260700.027.812053
AGAGTAC266450.026.49752410-11
CATGGGG151050.026.3436364
TACATGG286750.026.2403092
GAGTACT119350.023.94645712-13
CAGAGTA284300.022.79400810-11
ATGGGGG84750.022.5286735
CATGGGT53350.019.7337274
AGTACTT158050.019.13668812-13
Produced by FastQC (version 0.11.5)