FastQCFastQC Report
Fri 10 Feb 2017
ERR1148032.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148032.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3603198
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146140.4055841505240622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82960.23023991465359384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71680.19893439106038582No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT37370.10371342346437804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109400.058.272821
GGTATCA55950.057.4496651
TCAACGC150250.042.2400324
ATCAACG153100.041.4537243
AACGCAG153100.041.4537246
CAACGCA152950.041.4166035
TATCAAC174450.036.6888162
ACGCAGA180300.035.2330177
CGCAGAG187350.033.84378
GCAGAGT199100.031.8463959
GTACATG183300.028.3170411
AGAGTAC189550.027.45734810-11
TACATGG189450.027.3473952
ACATGGG185100.027.2819613
CATGGGG107750.026.110684
AGTACTT86200.026.04857812-13
GAGTACT81200.025.16199312-13
CAGAGTA195050.024.56370710-11
ATGGGGG54050.022.88985
GTACTTT89450.022.67505514-15
Produced by FastQC (version 0.11.5)