FastQCFastQC Report
Fri 10 Feb 2017
ERR1148031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748499
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67920.907416041972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42870.572746256174023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.4547768266891472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12110.16179046331391223No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC10650.14228475923147527No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.1369407307157391No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG8450.11289260239492638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.060.8029981
GTATCAA40050.054.4076731
TATCAAC52900.041.504112
TCAACGC52600.041.2828334
CAACGCA53650.040.4748735
ATCAACG54050.040.1753353
AACGCAG54050.040.1753356
ACGCAGA62850.034.5501487
CGCAGAG64800.033.5104458
GCAGAGT68500.031.7003949
ATATGTA6350.028.1273731
GTACATG57200.026.6456031
TACCGTG900.0095327226.441127
CTAGACA1358.249469E-526.4411184
GAGTACT30200.026.39734312-13
ATGGGGG24000.026.275865
ATGTAAG7700.026.2694233
AGTACAC17100.026.093213
CATGGGG36600.026.007664
ACATGGG57450.025.8888243
Produced by FastQC (version 0.11.5)