FastQCFastQC Report
Fri 10 Feb 2017
ERR1148030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775799
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211201.1893237917129134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121890.686395250813859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107300.6042350513768732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32800.1847055888645055No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT19940.1122874829865317No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT18080.10181332459360548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194100.043.511991
ATGGGTG16700.035.6137355
GGTATCA127050.035.181741
AACGCAG241600.034.7839786
TATCAAC242750.034.7661972
CATGGGT28800.034.2806934
ATCAACG255300.033.0571673
CAACGCA259300.032.3637285
TCAACGC260500.032.3287964
GGTGACC56150.031.9883029
CATGGGG111200.031.5559484
GGGTGAC57550.031.313488
ATGGGGT85500.030.3287435
ACGCAGA277150.030.2149457
CGCAGAG281400.029.779748
TGGGGTG71350.029.758316
GGGGTGA61550.029.5683697
ACATGGG231800.029.5578633
GTACATG241000.029.3641131
TACATGG242500.029.3082712
Produced by FastQC (version 0.11.5)