FastQCFastQC Report
Fri 10 Feb 2017
ERR1148029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092398
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185090.5985322717192288No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA127310.41168698207669263No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC115020.371944361624862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98510.3185553735321262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88140.2850215269832667No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG66680.21562554367193357No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA54210.17530085066669943No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC43720.14137895574890424No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC35360.11434491937971762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG112150.030.0191044
ACATGGG235100.027.9822773
GAGTACT109900.025.51905312-13
AGTACTT113900.025.51064312-13
CATGGGT38750.025.0205294
ATGGGTG26300.023.973465
ATGGGGT76900.023.8234675
GGGGTGA47350.023.6166577
GTACTTT125750.022.32618714-15
TGGGGTG65000.021.6878036
TACTTTT140000.020.86094914-15
ATGGGAG26400.020.503035
TACATGG429100.020.1003722
ACTTTTT142350.019.84799816-17
GTACATG511400.018.3707681
GGTGACC60650.018.3396649
GTATCAA438000.018.2414531
GGGTGAC61100.018.2045948
GTATTAG12900.017.9993131
ATGGGGG41550.017.89455
Produced by FastQC (version 0.11.5)