FastQCFastQC Report
Fri 10 Feb 2017
ERR1148028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294506
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246410.7479421801022673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136280.41365837548937534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123260.3741380346552715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA215200.044.457761
AACGCAG248350.038.0789386
GGTATCA133700.035.1333471
TATCAAC273250.034.9142462
ATCAACG274300.034.5849153
TCAACGC274050.034.5296554
CAACGCA277500.034.0789345
ACGCAGA282750.033.3620267
CGCAGAG286950.032.873728
ACATGGG240050.032.011993
CATGGGG142800.031.9045334
GTACATG253850.031.5910721
TACATGG255150.031.120332
GCAGAGT306650.030.8006159
CATGGGT36200.027.7670654
GAGTACT136050.027.3451712-13
AGAGTAC292750.026.89942210-11
ATGGGGG69300.026.5202225
AGTACTT151000.026.39062912-13
ATGGGGT71500.025.7873975
Produced by FastQC (version 0.11.5)