FastQCFastQC Report
Fri 10 Feb 2017
ERR1148027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379013
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218890.6477927134343667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124730.3691314593936158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105640.31263567201428344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA170700.053.633041
GGTATCA93250.046.2167471
AACGCAG215900.041.9856876
TATCAAC221550.041.077262
ATCAACG225200.040.3574643
TCAACGC224750.040.3324134
CAACGCA227250.039.9148835
ACGCAGA248600.036.4869547
CGCAGAG253650.035.7839748
GCAGAGT275500.033.0107049
GTACATG232250.032.4223481
ACATGGG229300.032.1133423
CATGGGG133350.031.535184
TACATGG239250.031.2510852
CATGGGT35350.028.772374
ATGGGGG62100.028.5426035
AGAGTAC264950.027.51175710-11
GAGTACT125200.026.91314912-13
AGTACTT136000.026.13171412-13
CAGAGTA267250.025.00485210-11
Produced by FastQC (version 0.11.5)