FastQCFastQC Report
Fri 10 Feb 2017
ERR1148026.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148026.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115817
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144910.35208076549564765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83490.20285158450922383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69030.16771882714902048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108100.059.308441
GGTATCA55550.056.2602581
TCAACGC146900.043.405084
CAACGCA148900.042.8220675
AACGCAG150750.042.414936
ATCAACG152450.041.8639143
TATCAAC173800.036.927942
ACGCAGA179250.035.73757
CGCAGAG187350.034.033668
GCAGAGT203250.031.4883049
GTACATG192400.030.4140991
TACATGG198150.029.448172
ACATGGG195300.029.1154293
AGAGTAC186350.027.62518910-11
AGTACTT83500.026.24935212-13
CATGGGG116450.025.4365044
CAGAGTA194650.024.50685310-11
GAGTACT80800.024.32919112-13
CATGGGT44200.022.4730364
GTACTTT87850.022.37676414-15
Produced by FastQC (version 0.11.5)