FastQCFastQC Report
Fri 10 Feb 2017
ERR1148025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891851
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121680.4207685665686095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77970.269619700323426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.19790092919725116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.065.8991241
GTATCAA91350.055.643021
TCAACGC125000.040.6833844
ATCAACG128200.039.714283
CAACGCA127850.039.6834345
AACGCAG129900.039.194546
TATCAAC134000.038.173512
ACGCAGA155150.032.8541347
CGCAGAG159850.031.9625558
GCAGAGT170850.029.800249
GTACATG158850.029.0386141
ACATGGG159800.027.9527233
TACATGG163100.027.8981842
CATGGGG103850.025.7158454
GAGTACT69100.025.3924712-13
AGAGTAC163000.025.37876310-11
CAGAGTA167650.024.63937410-11
GTAAGTA26350.023.701572
ATGGGGG63050.023.678255
AGTACTT73700.023.16196412-13
Produced by FastQC (version 0.11.5)