FastQCFastQC Report
Fri 10 Feb 2017
ERR1148024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532310
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121780.7947477990745997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84860.5538043868407828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68620.44782061071193163No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.14416142947575883No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16530.10787634355972356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48350.062.549661
GTATCAA90050.055.5332831
AACGCAG116050.043.0065276
TCAACGC119050.041.8728144
TATCAAC119150.041.837672
ATCAACG119050.041.8228453
CAACGCA121050.041.1809845
ACGCAGA140050.035.636617
CGCAGAG141550.035.1328938
GCAGAGT151350.032.9366269
GTACATG126000.030.5224741
ACATGGG127050.029.7783073
TACATGG129800.029.4223842
CATGGGG72900.029.049594
ATATAGG7650.027.215992
GAGTACT72200.027.18906412-13
AGAGTAC148250.026.86416810-11
CAGAGTA150500.026.46254510-11
GATATAG9000.026.4591121
ATGGGGG39150.025.9825555
Produced by FastQC (version 0.11.5)