FastQCFastQC Report
Fri 10 Feb 2017
ERR1148023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597048
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317571.2228114382175455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169770.653703743635081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161140.6204737070704892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51300.1975319670641436No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA27290.10508084563704637No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.10407970896186747No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26570.10230846715193558No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26130.10061423585547899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA281700.041.0472641
AACGCAG341900.033.7796826
TATCAAC350750.032.978872
CATGGGG154700.032.099754
ATCAACG363400.031.862993
CAACGCA365350.031.644095
TCAACGC367750.031.4860924
CATGGGT40650.029.9915454
ACGCAGA388300.029.8657027
ACATGGG303850.029.6719073
CGCAGAG395550.029.4085068
GGTATCA189950.028.8548361
ATGGGTG25150.028.8487725
AGTACTT176250.028.20867212-13
TACATGG337800.028.045942
GTACATG346750.027.6831911
GCAGAGT423450.027.4849019
GAGTACT172550.026.59049612-13
ATGGGAG20250.026.1378865
ATGGGGT106100.025.8399125
Produced by FastQC (version 0.11.5)