FastQCFastQC Report
Fri 10 Feb 2017
ERR1148020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303906
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250620.758556690172178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124340.376342426207041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123840.374829065960109No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC59850.1811492215577562No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38270.11583259330017258No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG37400.11319934647051097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA211750.052.1435241
AACGCAG272600.040.3436666
TATCAAC279100.039.4473152
GGTATCA125100.038.9678541
ATCAACG291450.037.7139633
CAACGCA291450.037.6731455
TCAACGC291500.037.6462824
ACGCAGA311700.035.2447437
CGCAGAG320750.034.2873958
GTACATG321250.033.888351
TACATGG330900.032.8946462
ACATGGG320250.032.8551143
CATGGGG202050.032.587654
GCAGAGT347250.031.7393079
GTAAGTA49350.030.9753932
CATGGGT42750.030.609124
ATATGTA29450.029.3064081
TATGTAA31500.028.3238032
ATGGGGG112950.028.1202585
TGTAAGT53050.028.0501061
Produced by FastQC (version 0.11.5)