FastQCFastQC Report
Fri 10 Feb 2017
ERR1148019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705359
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170340.4597125406741964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98150.2648866142255042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81680.22043747987711854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103500.060.9617421
GGTATCA50250.060.057011
TCAACGC137800.045.1506084
CAACGCA141600.043.980945
ATCAACG143700.043.2968253
AACGCAG144500.043.0571176
TATCAAC148700.042.2032662
ACGCAGA170950.036.499557
CGCAGAG178150.035.0244068
GCAGAGT197150.031.6791579
GTACATG170700.030.093251
TACATGG176000.028.694562
ACATGGG177000.027.993223
AGAGTAC183100.027.54790910-11
GAGTACT88350.025.88635412-13
CATGGGG105750.025.7049624
AGTACTT95150.025.38039412-13
CAGAGTA192200.024.09274910-11
GTACTTT99000.023.1316514-15
ATGGGGG58600.021.5188645
Produced by FastQC (version 0.11.5)