FastQCFastQC Report
Fri 10 Feb 2017
ERR1148018.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148018.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141106
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124920.3976943153144148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71420.22737214216903218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60300.1919705988909639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40150.063.6015551
GTATCAA84350.060.4066351
TCAACGC108600.046.393714
CAACGCA111500.045.1870575
ATCAACG113750.044.293253
AACGCAG115200.043.8906486
TATCAAC126250.040.331832
ACGCAGA139250.036.3102537
CGCAGAG145250.034.646538
GTACATG153900.032.5663761
GCAGAGT157100.032.2224779
TACATGG158500.031.3749122
ACATGGG154450.031.2347813
CATGGGG103100.028.9057734
ATGGGGG61300.026.9767655
AGAGTAC149400.026.67655410-11
GAGTACT65950.026.42763712-13
TGGGGGG48500.025.7562796
CAGAGTA153800.024.94645910-11
GTACTTT73700.023.81003614-15
Produced by FastQC (version 0.11.5)