FastQCFastQC Report
Fri 10 Feb 2017
ERR1148017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492126
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165341.108083365613896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97200.651419518190823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80520.5396327119827682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.1739799453933515No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18330.12284485358475088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128000.047.0411261
GGTATCA74550.040.463881
AACGCAG155300.038.4485746
TATCAAC161200.037.4471632
ATCAACG161750.037.0992243
TCAACGC162300.036.9735034
CAACGCA165150.036.335455
ACGCAGA176650.033.868997
CGCAGAG180250.033.2255488
GCAGAGT189900.031.5371519
CATGGGG76500.029.386534
ACATGGG141650.029.1380713
GTACATG152500.028.2472321
GAGTACT92950.027.89684312-13
TACATGG154450.027.7629282
AGTACTT95300.027.3961512-13
ATTAACG1107.773108E-427.0330622
CATACGT450.00953941326.43986118-119
ATGGGGG33150.026.3725285
TAACGCA1608.774783E-626.0193214
Produced by FastQC (version 0.11.5)