FastQCFastQC Report
Fri 10 Feb 2017
ERR1148016.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148016.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703486
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207720.5608769683481996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132370.3574200091481377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99510.26869279376241734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74300.054.739981
GTATCAA137750.053.777611
TCAACGC177300.041.533424
ATCAACG179600.040.9684143
AACGCAG181850.040.494236
CAACGCA182050.040.4824145
TATCAAC192700.038.28112
ACGCAGA210000.035.09447
CGCAGAG217850.033.80258
GCAGAGT234500.031.4531849
GTACATG191650.027.4362411
GAGTACT106700.027.09303312-13
AGAGTAC220600.026.6806310-11
TACATGG197950.026.657082
ACATGGG195200.026.4804733
CATGGGG110250.025.1955624
CAGAGTA229600.024.97416310-11
AGTACTT114000.024.70591212-13
GTACTTT127150.022.78234114-15
ATGGGGG56400.022.3584965
Produced by FastQC (version 0.11.5)