FastQCFastQC Report
Fri 10 Feb 2017
ERR1148015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7981983
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481270.6029454084279559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253470.31755266830310214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210920.26424511302517184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA321700.062.0107231
GGTATCA150050.060.4814261
AACGCAG431800.045.9098436
TCAACGC433200.045.8026584
ATCAACG438500.045.2903253
CAACGCA442500.044.853475
TATCAAC453450.043.9756362
ACGCAGA503000.039.482227
CGCAGAG514850.038.5388228
GCAGAGT560550.035.5666359
GTACATG495250.032.104051
TACATGG505600.031.2270762
ACATGGG506250.030.7936063
CATGGGG291500.029.3613054
AGAGTAC532350.029.3170110-11
GAGTACT251150.027.42385312-13
CAGAGTA550550.026.42486810-11
AGTACTT265350.026.23647512-13
ATGGGGG152450.025.8275785
CATGGGT109600.025.6144244
Produced by FastQC (version 0.11.5)