FastQCFastQC Report
Fri 10 Feb 2017
ERR1148014.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148014.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4178498
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246090.5889436826342863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147170.3522078986276887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110990.26562176169523116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83450.063.366471
GTATCAA162300.056.9804461
AACGCAG213800.043.2048236
TCAACGC218000.042.3724374
ATCAACG219750.042.0891383
CAACGCA222250.041.5889325
TATCAAC232300.040.000732
ACGCAGA247150.037.3748447
CGCAGAG254200.036.3850828
GCAGAGT275300.033.7044269
GTACATG245100.031.5358311
CATGGGG149850.031.039774
TACATGG250150.030.8919092
ACATGGG250350.030.411013
ATGGGGG79200.028.5382235
AGAGTAC260450.028.36388410-11
CAGAGTA269550.026.99809610-11
GAGTACT125900.026.62175812-13
AGTACTT129600.025.79287712-13
GTACTTT139300.024.12491614-15
Produced by FastQC (version 0.11.5)