FastQCFastQC Report
Fri 10 Feb 2017
ERR1148010.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148010.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4128758
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518811.256576432912755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302870.7335620058138549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261210.6326599912128538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92680.22447428500289918No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC55030.1332846342653166No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49500.11989077587012849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA350250.054.0754131
GGTATCA189850.050.430091
AACGCAG418400.044.98036
TATCAAC458300.041.262452
ATCAACG459250.041.0052343
TCAACGC459950.040.942834
CAACGCA465650.040.4544225
ACGCAGA492450.038.26497
CGCAGAG497250.037.907498
GCAGAGT525600.035.930729
CATGGGG247550.033.8552174
GTACATG436400.033.4677961
ACATGGG429350.033.2073443
ATGGGGG124650.033.1642465
TACATGG443800.032.933072
CATGGGT61750.030.3424854
AGAGTAC521150.029.4408510-11
GGGGTGA67300.028.105397
TGGGGGG110550.027.9783526
AGTACTT272900.027.5717612-13
Produced by FastQC (version 0.11.5)