FastQCFastQC Report
Fri 10 Feb 2017
ERR1148009.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148009.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577565
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293330.8199152216661333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173570.4851623939746727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145670.4071763895275139No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.12547640643845745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92600.061.154221
GTATCAA179450.057.937221
AACGCAG232300.044.375036
TCAACGC237950.043.471354
ATCAACG238950.043.239643
CAACGCA242250.042.6506165
TATCAAC249350.041.6345862
ACGCAGA271000.038.257577
CGCAGAG276750.037.4626968
GCAGAGT296600.035.1761059
GTACATG242100.034.5081
TACATGG248550.033.749982
ACATGGG246850.033.3256263
CATGGGG131850.030.496914
AGAGTAC289500.029.00157510-11
ATGGGGG70400.027.9669195
CATGGGT46350.027.4633374
CAGAGTA294500.027.34781510-11
GAGTACT158150.027.13666212-13
AGTACTT163300.026.6268912-13
Produced by FastQC (version 0.11.5)