FastQCFastQC Report
Fri 10 Feb 2017
ERR1148008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160473
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255650.8088979086358276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148260.4691069975918161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131460.4159503972981259No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.12586723569541647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA172700.054.958621
GGTATCA91400.050.4236871
AACGCAG215600.043.7004286
TCAACGC226700.041.613184
ATCAACG227650.041.387273
CAACGCA230750.040.9859165
TATCAAC233750.040.621572
ACGCAGA249450.037.8418777
CGCAGAG254000.037.187428
GCAGAGT271050.034.8701449
GTACATG216650.031.1394581
TATCACG2750.030.2880132
TACATGG222150.030.1287862
ACATGGG219050.029.9238553
CATGGGG127350.029.098354
AGAGTAC262100.028.8895410-11
CAGAGTA266900.026.35310710-11
AGTACTT147100.026.30351812-13
GAGTACT141100.026.09413512-13
ATGGGGG60200.023.713415
Produced by FastQC (version 0.11.5)