FastQCFastQC Report
Fri 10 Feb 2017
ERR1148006.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148006.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865721
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255690.6614290063871655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146480.37892025834249293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121130.31334387556680887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39510.10220603090600691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82450.059.2151
GTATCAA166150.059.0203551
AACGCAG217750.044.77046
TCAACGC219700.044.3459554
ATCAACG221800.043.9272233
CAACGCA223600.043.572485
TATCAAC234250.041.747622
ACGCAGA253050.038.5955287
CGCAGAG257950.037.839318
GCAGAGT279000.035.0910039
GTACATG244200.032.013091
TACATGG248800.031.2967172
ACATGGG247250.030.9856743
CATGGGT49650.028.751614
CATGGGG142750.028.625354
AGAGTAC269450.028.35476910-11
GAGTACT132400.026.81985912-13
CAGAGTA275250.026.67681310-11
AGTACTT138700.025.66598112-13
ATGGGGG74300.024.9767515
Produced by FastQC (version 0.11.5)