FastQCFastQC Report
Fri 10 Feb 2017
ERR1148005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4653539
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266030.5716724411249159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131260.2820648972749557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122290.26278924491661076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA173000.062.2511861
GGTATCA82050.053.9820631
TCAACGC231100.046.0148354
AACGCAG231600.045.8898166
ATCAACG232950.045.6494033
CAACGCA235900.045.053335
TATCAAC251400.042.6373142
ACGCAGA265900.040.059697
CGCAGAG272450.039.096618
GCAGAGT296450.036.0718579
GTACATG280500.033.533541
TACATGG286700.032.6155852
ACATGGG283900.032.345383
CATGGGG162150.030.9934624
AGAGTAC283550.029.88928410-11
ATGGGGG81450.029.1348045
CATGGGT67250.027.5041624
AGTACTT136950.026.70812412-13
GAGTACT129250.026.25157712-13
CAGAGTA291050.026.0845610-11
Produced by FastQC (version 0.11.5)