FastQCFastQC Report
Fri 10 Feb 2017
ERR1148004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2902718
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171680.5914456726419859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100670.34681288364904894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78290.26971273130906964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.068.0336151
GTATCAA108000.065.5545351
TCAACGC149350.047.3125954
ATCAACG150900.046.905453
AACGCAG151200.046.6943666
CAACGCA153050.046.2465325
TATCAAC159150.044.8157272
ACGCAGA178700.039.675037
CGCAGAG182350.038.880888
GCAGAGT198850.035.7144789
GTACATG189650.033.2810971
TACATGG194400.032.221822
ACATGGG191000.032.1997683
CATGGGG113650.031.7152924
AGAGTAC190450.029.18528410-11
ATGGGGG67500.029.1668665
GAGTACT84000.027.2613312-13
CAGAGTA195050.026.69781910-11
AGTACTT88900.026.32743512-13
CATGGGT46000.026.2484594
Produced by FastQC (version 0.11.5)