FastQCFastQC Report
Fri 10 Feb 2017
ERR1148003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792798
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149000.5335151342846851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86120.30836458633957775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70080.2509311450380586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94600.057.395121
GGTATCA47350.054.9455761
TCAACGC122600.043.9076734
AACGCAG122900.043.8004956
CAACGCA124600.043.20295
ATCAACG124800.043.0860023
TATCAAC134750.040.130972
ACGCAGA146850.036.5759937
CGCAGAG151300.035.500238
GCAGAGT165350.032.483739
GTACATG141700.031.4270081
TACATGG146000.030.4377332
ACATGGG142900.030.094593
CATGGGG82950.028.1811374
AGAGTAC155800.026.99195310-11
GAGTACT80150.026.2713312-13
ATGGGGG44750.025.65355
AGTACTT84800.025.28667812-13
CAGAGTA163400.024.48062910-11
CATGGGT30650.022.7058474
Produced by FastQC (version 0.11.5)