FastQCFastQC Report
Fri 10 Feb 2017
ERR1148001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417679
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240480.703635420412508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139670.40866915822112027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123500.3613563473924848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40040.11715553157566874No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35230.10308165278248776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82400.057.5859221
GTATCAA156450.055.521981
AACGCAG200350.042.868396
TCAACGC204500.042.027534
ATCAACG207650.041.447273
CAACGCA208500.041.1641925
TATCAAC220350.039.305392
ACGCAGA234200.036.87567
CGCAGAG239850.035.982148
GCAGAGT260450.033.2731979
GTACATG212500.031.6035181
TACATGG218650.030.6876372
ACATGGG215400.030.457143
CATGGGG120250.029.0838264
AGAGTAC247200.028.8008310-11
GAGTACT129950.026.9586612-13
ATGGGGG58900.026.8612165
CAGAGTA254650.026.40500310-11
AGTACTT137300.025.9920212-13
TATAACG3051.0913936E-1125.3540482
Produced by FastQC (version 0.11.5)