FastQCFastQC Report
Fri 10 Feb 2017
ERR1148000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629328
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86130.5286228432826294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53910.3308726051476437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41190.2528036098317834No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16810.10317136880971788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.064.300891
GTATCAA56100.057.4226841
TCAACGC74200.043.607784
ATCAACG75800.042.765773
AACGCAG75950.042.759626
CAACGCA76500.042.3744475
TATCAAC83100.039.228512
ACGCAGA90050.036.262497
CGCAGAG93650.034.9320378
GTACATG90500.033.3586461
TACATGG92500.032.5410162
GCAGAGT100800.032.454229
ACATGGG91400.032.147693
ATGGGGG33450.031.4735775
CATGGGG56200.031.0098954
ATATGTA11850.027.1346651
AGAGTAC96000.026.82783710-11
TATGTAA12200.026.8179242
CAGAGTA99500.025.76458710-11
TGGGGGG29450.025.6500026
Produced by FastQC (version 0.11.5)