FastQCFastQC Report
Fri 10 Feb 2017
ERR1147999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2183579
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114520.5244600722025629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69500.3182847975731586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54930.25155948101717407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.058.891881
GTATCAA77750.055.596041
AACGCAG103100.041.998396
TCAACGC103400.041.8190164
ATCAACG104200.041.4979443
CAACGCA105050.041.162175
TATCAAC115300.037.76432
ACGCAGA119000.036.436827
CGCAGAG123150.035.2572448
GCAGAGT133200.032.6863759
GTACATG113450.031.646121
TACATGG115900.030.6914542
ACATGGG115950.029.854663
AGAGTAC125950.027.3426310-11
CATGGGG62050.026.8396074
AGTACTT68250.026.36226312-13
GAGTACT64400.026.32200212-13
CAGAGTA131050.025.9608510-11
CATGGGT26400.023.8815144
GTACTTT73150.023.01081714-15
Produced by FastQC (version 0.11.5)