FastQCFastQC Report
Fri 10 Feb 2017
ERR1147997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448768
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159860.6528180701479275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82400.3364957398985939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77290.3156281036014845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97950.060.7851521
GGTATCA48250.052.8139231
AACGCAG130100.045.3021936
TCAACGC135800.043.356914
ATCAACG136750.043.0557063
CAACGCA137850.042.7984245
TATCAAC143400.041.2714772
ACGCAGA154500.038.3016477
CGCAGAG159050.037.205948
GCAGAGT172650.034.2751549
GTACATG151750.033.977481
TACATGG156500.032.8379062
ACATGGG155150.032.4295923
CATGGGG89600.031.3961224
AGAGTAC163350.029.8732410-11
ATGGGGG43350.028.5362645
AGTACTT87950.026.20346612-13
CAGAGTA168050.025.26868210-11
GAGTACT84600.025.16723812-13
TATCACG1901.1724314E-625.0445062
Produced by FastQC (version 0.11.5)