FastQCFastQC Report
Fri 10 Feb 2017
ERR1147995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067608
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127340.6158807665669701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73880.3573211169622095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59970.2900453083950149No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.10383979942039302No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.10050260977902967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87850.054.765841
GGTATCA48100.048.6505361
AACGCAG114900.041.722646
TCAACGC115950.041.139634
ATCAACG116800.040.7893183
CAACGCA117950.040.643765
TATCAAC122250.039.174982
ACGCAGA133350.036.0391967
CGCAGAG137800.035.0048648
GCAGAGT150300.032.172769
GTACATG129600.030.3694251
CATGGGG74100.030.2607634
TACATGG132300.029.9036062
ACATGGG130300.029.7618483
ATGGGGG44000.028.3872535
AGAGTAC144600.026.90086410-11
GAGTACT70900.026.04786312-13
AGTACTT74650.025.89466512-13
TGGGGGG38850.025.5671446
TAACGCA1951.4704638E-624.4012534
Produced by FastQC (version 0.11.5)