FastQCFastQC Report
Fri 10 Feb 2017
ERR1147994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206174
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114410.518590102140629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71950.32613021457056424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54790.24834849835053807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.060.060521
GTATCAA80500.058.058411
AACGCAG109000.042.616976
TCAACGC108450.042.6137244
ATCAACG109750.042.1089633
CAACGCA110350.042.0417025
TATCAAC118500.039.106242
ACGCAGA129050.035.9957357
CGCAGAG133150.034.9766858
GTACATG129700.033.2345621
GCAGAGT141600.033.015469
TACATGG131600.032.5464442
ACATGGG131950.031.8238963
CATGGGG74400.029.9789474
AGAGTAC135750.027.80032210-11
ATGGGGG39550.027.069745
GAGTACT66200.026.50464812-13
CATGGGT31850.025.7707884
CAGAGTA138900.025.62830710-11
TGGGGGG31500.025.1130316
Produced by FastQC (version 0.11.5)