FastQCFastQC Report
Fri 10 Feb 2017
ERR1147993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937970
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122040.6297311103887057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73670.380140043447525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60290.3110987270184781No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20350.10500678545075517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39750.059.182391
GTATCAA76300.055.8102571
TCAACGC104250.040.7406274
ATCAACG104250.040.6835673
AACGCAG104350.040.4735646
CAACGCA106500.039.8240555
TATCAAC111450.038.220342
ACGCAGA121750.034.786977
CGCAGAG124000.034.1557548
GCAGAGT135600.031.146149
GTACATG110700.030.2357831
TACATGG114200.029.3815422
ACATGGG112400.029.2131443
CATGGGG66850.027.4065824
AGAGTAC130150.027.08006910-11
GAGTACT67000.025.74712612-13
ATGGGGG38700.025.5154195
AGTACTT68900.025.1666212-13
CAGAGTA131650.024.98675310-11
TATACCG1951.4689485E-624.4039965
Produced by FastQC (version 0.11.5)