FastQCFastQC Report
Fri 10 Feb 2017
ERR1147992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824100
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141260.5001947523104706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78240.2770440140221664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64870.22970149782231508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108300.052.8348661
GGTATCA59050.051.2235261
AACGCAG137000.041.6360786
TCAACGC141250.040.172764
ATCAACG142250.039.9746863
CAACGCA143650.039.667215
TATCAAC151500.037.8534432
ACGCAGA160450.035.5879757
CGCAGAG164000.034.8538938
GTACATG169300.034.044271
TACATGG174350.033.1312832
ACATGGG173200.032.7970623
GCAGAGT179950.031.9629159
CATGGGT39700.031.7626784
CATGGGG93000.030.1237964
ATGGGGG47200.028.101835
AGAGTAC170650.027.1520710-11
ATGGGTA19800.026.4356025
GAGTACT77700.025.22354512-13
CAGAGTA176000.024.92419210-11
Produced by FastQC (version 0.11.5)