FastQCFastQC Report
Fri 10 Feb 2017
ERR1147991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048036
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93450.45629080738815136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.26830583056157215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.21054317404576875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68500.050.8494341
GGTATCA39250.050.5155831
AACGCAG84450.041.3441166
TCAACGC86850.040.1331334
ATCAACG87800.039.6311453
CAACGCA88300.039.4067345
TATCAAC95950.036.458862
ACGCAGA99350.035.2632837
CGCAGAG101650.034.289848
GTACATG102850.033.519311
TACATGG106850.031.9044172
CATGGGG57200.031.0921254
GCAGAGT112600.031.0080879
ACATGGG107000.030.9076713
GAGTACT50300.026.60662712-13
ATGGGGG29250.026.4358185
AGAGTAC106900.025.6784810-11
AGTACTT54450.025.4526712-13
CAGAGTA110200.024.77458410-11
GCACCGT4100.024.6626856
Produced by FastQC (version 0.11.5)