FastQCFastQC Report
Fri 10 Feb 2017
ERR1147990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290670
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129480.5652494684961169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72430.3161956982018362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60690.26494431760140047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96500.055.8232081
GGTATCA51700.049.9680861
AACGCAG126850.042.1513866
ATCAACG128100.041.925793
TCAACGC129100.041.5088964
CAACGCA132100.040.5212025
TATCAAC136450.039.5379642
ACGCAGA146900.036.3982547
CGCAGAG151800.035.2233438
GTACATG155350.034.5995181
ACATGGG155400.033.9863553
TACATGG160600.033.555462
GCAGAGT162550.033.0036669
CATGGGG78150.032.5729754
ATGGGGG43150.030.8752065
AGAGTAC157150.027.32533310-11
CATGGGA63700.026.6101934
CATGGGT28700.026.1114674
TATGCGT1150.001007475725.8591824
GAGTACT74250.025.47260312-13
Produced by FastQC (version 0.11.5)