FastQCFastQC Report
Fri 10 Feb 2017
ERR1147989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601760
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96380.6017131155728699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.3182748976126261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.2879332733992608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69850.051.568191
GGTATCA39550.045.9141241
AACGCAG85300.041.562046
ATCAACG92700.038.4367523
TCAACGC92800.038.3312344
CAACGCA93600.038.0036165
TATCAAC99300.036.245992
GTACATG102750.036.099331
ACGCAGA99750.035.6009067
TACATGG104750.035.1552732
ACATGGG102450.034.9529763
CGCAGAG102650.034.768988
GCAGAGT112250.031.9543919
CATGGGG59900.031.8770264
CATGGGT21250.030.5117554
ATGGGGG30450.030.2791755
GTAAGTA13650.029.2008862
GGGTTAG5150.028.9018551
AGAGTAC106800.027.79258310-11
AGTACTT55200.026.02421612-13
Produced by FastQC (version 0.11.5)