FastQCFastQC Report
Fri 10 Feb 2017
ERR1147988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416392
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132430.5480484954427923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76550.3167946260374972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64060.2651059927362779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97000.053.531371
GGTATCA51800.048.856551
AACGCAG120200.043.1023256
TCAACGC125700.041.216394
ATCAACG126800.040.8588333
CAACGCA128000.040.522255
TATCAAC135550.038.4042
ACGCAGA139700.037.0858967
CGCAGAG142550.036.4696248
GTACATG143800.033.127961
GCAGAGT157750.032.9932989
TACATGG147300.032.3517462
ACATGGG143500.032.290343
CATGGGT32150.031.0824584
CATGGGG75900.028.7614884
AGAGTAC149000.028.16422510-11
ATGGGGG37300.027.269325
AGTACTT76950.025.04513712-13
CAGAGTA155200.024.91200610-11
GAGTACT73850.024.84801712-13
Produced by FastQC (version 0.11.5)