FastQCFastQC Report
Fri 10 Feb 2017
ERR1147987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611339
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115320.7156780789144929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68390.4244296203343927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55620.34517876126625124No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC18910.11735581401554857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18820.11679727233065171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84200.057.000721
GGTATCA44200.051.7328451
AACGCAG108450.044.0972986
TCAACGC111500.042.891054
ATCAACG111900.042.737733
CAACGCA113850.042.0579725
TATCAAC114700.041.9070742
ACGCAGA124950.038.3693357
CGCAGAG126400.037.976248
GTACATG123950.036.0786931
TACATGG125950.035.3771632
ACATGGG124900.035.3367773
GCAGAGT137300.035.091349
ATGGGGG37350.034.8769875
CATGGGG69550.032.841244
CATGGGT24500.031.8046534
TGGGGGG33400.028.8505466
AGAGTAC131900.028.5910410-11
GTAAGTA16650.027.1543082
GAGTACT64850.027.05811312-13
Produced by FastQC (version 0.11.5)