FastQCFastQC Report
Fri 10 Feb 2017
ERR1147986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396571
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63650.45575914149728153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37510.26858641630106883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30540.21867846317874276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56400.051.8416141
TATCACG952.6357156E-950.1018262
GGTATCA32050.047.7508661
AACGCAG70750.040.7778856
TAACGCA2200.040.558144
TCAACGC71700.039.905744
ATCAACG72050.039.8770073
CAACGCA72500.039.4653975
TATCAAC79600.036.624372
TATAACG2150.035.9742742
GTACATG88000.035.323521
ACGCAGA83350.034.684877
ACATGGG87350.034.1180533
TACATGG90550.034.1009182
CGCAGAG86550.033.4024738
GCAGAGT92000.031.5530559
CATGGGG47400.030.3701154
CATGGGT21100.029.6016714
TAGTACG1107.763298E-427.038764
ATGGGGG26200.027.0181185
Produced by FastQC (version 0.11.5)