FastQCFastQC Report
Fri 10 Feb 2017
ERR1147985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541781
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127700.8282628985569286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73430.47626738168390975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65040.42184979578811777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20720.13439003334455413No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.1055273090017324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90550.055.3715321
GGTATCA47700.050.0597721
AACGCAG109500.045.305386
ATCAACG117800.042.214233
TCAACGC118100.042.056634
TATCAAC120700.041.5530322
CAACGCA120150.041.339065
ACGCAGA127350.039.0952877
CGCAGAG130000.038.2983448
GCAGAGT137700.036.2863439
TATCACG851.7115545E-434.997212
GTACATG119250.033.9058231
TACATGG122300.033.0799522
CATGGGG66350.032.63314
ACATGGG122150.032.5297623
ATGGGGG29150.030.6091025
AGAGTAC134250.028.68952410-11
CATGGGT25300.028.2136084
CAGAGTA135950.027.12753710-11
GTAAGTA11750.026.3298172
Produced by FastQC (version 0.11.5)