FastQCFastQC Report
Fri 10 Feb 2017
ERR1147984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658309
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94100.5674455122658081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57240.34517089396487627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.2780543312494837No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.10558948905179916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69850.052.9395981
GGTATCA38500.052.8957561
AACGCAG89650.041.336596
TCAACGC92500.039.8700564
ATCAACG92650.039.8055083
CAACGCA93300.039.528195
TATCAAC97250.037.9907152
ACGCAGA105900.035.1621367
CGCAGAG108200.034.41478
GCAGAGT117400.031.667149
GTACATG103900.031.521191
TACATGG107100.030.6081852
ACATGGG104300.030.4546683
CATGGGG55200.026.6167354
AGAGTAC111300.026.29461710-11
CATGGGT21750.026.254814
GAGTACT56300.025.40993712-13
TATCACG1651.1110398E-525.2400152
CAGAGTA114600.025.12219610-11
AGTACTT57850.024.72911812-13
Produced by FastQC (version 0.11.5)