FastQCFastQC Report
Fri 10 Feb 2017
ERR1147983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525720
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130240.5156549419571449No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71470.28296881681263164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60490.23949606448854188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92650.054.2969061
GGTATCA50150.052.8266261
AACGCAG118550.042.093976
TCAACGC120800.041.556124
ATCAACG121700.041.2488063
CAACGCA123950.040.3080945
TATCAAC131700.038.3456232
ACGCAGA139450.035.827817
CGCAGAG143750.034.7147188
GTACATG141650.033.496971
TACATGG144550.032.3443452
ACATGGG142300.031.850013
GCAGAGT157750.031.7092639
CATGGGG77200.029.4310384
ATGGGGG39150.028.106035
CATGGGT29150.027.1376724
AGAGTAC150300.026.51400410-11
TATAACG1859.289215E-725.722442
GAGTACT76650.025.29676412-13
CAGAGTA156200.024.44632110-11
Produced by FastQC (version 0.11.5)