FastQCFastQC Report
Fri 10 Feb 2017
ERR1147981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112251
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158760.7516152199714902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83330.3945080390540707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76430.36184146675750184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.11007214578191701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144200.041.9182361
ATGGGGG48850.039.4491355
CATGGGG96100.037.011344
AACGCAG173000.034.5523036
TGGGGGG42050.033.3812836
ACATGGG184200.032.8412133
TCAACGC182650.032.791924
ATCAACG183850.032.5478443
CAACGCA184950.032.3841255
TATCAAC186650.032.2235032
GTACATG196150.031.8181691
TACATGG195600.031.752742
ACGCAGA195200.030.6226867
CGCAGAG197950.030.2573578
GGTATCA92500.029.615451
GCAGAGT210300.028.6501719
AGTACTT91250.026.46367612-13
CATGGGT29850.025.9033584
ATGGGAG20600.025.4081175
GAGTACT87850.025.11823812-13
Produced by FastQC (version 0.11.5)