FastQCFastQC Report
Fri 10 Feb 2017
ERR1147980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680132
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89880.534957967588261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.3168798642011461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.271466765706504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73700.049.909631
GGTATCA43050.045.763391
AACGCAG89800.040.5949976
TCAACGC95450.038.0674444
CAACGCA96700.037.7598535
ATCAACG97450.037.347193
CGTATAC801.2014213E-437.1679343
ACGCAGA103100.035.4158867
TATCAAC103550.035.3236122
TATAACG1209.321484E-734.6942022
CGCAGAG105650.034.5610778
GTACATG102950.033.6480561
ACATGGG103300.033.0445563
TACATGG105600.032.722942
GCAGAGT113750.032.3091439
CATGGGG53800.030.3975664
ATGGGGG25550.028.6287615
AGAGTAC109600.027.94377510-11
CATGGGT23850.027.6772524
AGTACTT58550.026.1032512-13
Produced by FastQC (version 0.11.5)