FastQCFastQC Report
Fri 10 Feb 2017
ERR1147979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632871
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82930.507878454574795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48530.29720657663710115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43720.26774925882081313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58400.055.5545431
GGTATCA32050.054.236381
AACGCAG77800.041.6671336
TCAACGC79450.040.5771984
CAACGCA81100.039.6783075
ATCAACG81500.039.629533
TATCAAC86950.037.216252
ACGCAGA90050.036.2631347
CGCAGAG91700.035.6754958
GTACATG92950.034.8405841
TACATGG94450.034.1980362
ACATGGG93350.033.7705573
GCAGAGT99450.033.0149779
CATGGGG48150.029.4006774
CATGGGT22500.029.3438554
AGAGTAC95350.028.47716910-11
GAGTACT45950.026.79547912-13
AGTACTT48500.025.57060612-13
CAGAGTA98650.025.47453910-11
ATGGGAG14650.024.7667435
Produced by FastQC (version 0.11.5)