FastQCFastQC Report
Fri 10 Feb 2017
ERR1147978.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147978.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468684
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105420.7177854460183403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63040.42922779849171094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50190.34173450517606235No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.11568179404146842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82500.052.7602961
GGTATCA45850.048.051421
AACGCAG99700.043.7970926
ATCAACG106900.040.7359433
TCAACGC107400.040.54634
CAACGCA109250.039.9141545
TATCAAC113300.038.7538832
ACGCAGA116700.037.366087
CGCAGAG118500.036.7984928
GCAGAGT127100.034.4490059
GTACATG109300.031.9787651
ACATGGG109800.030.8828183
TACATGG111600.030.8676192
CATGGGG59700.029.6952064
CATGGGT22300.028.8113274
ATGGGGG29650.028.4910245
AGAGTAC122900.028.29287310-11
AGTACTT63700.026.5230412-13
CAGAGTA125700.026.29015510-11
GAGTACT61350.026.22992912-13
Produced by FastQC (version 0.11.5)