FastQCFastQC Report
Fri 10 Feb 2017
ERR1147977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438908
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163620.6708740141079532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97740.4007531239390744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81290.3333049053100814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25840.10594905588894703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122350.054.854111
GGTATCA65600.051.9255871
AACGCAG151600.044.0252466
TCAACGC158300.042.1243174
ATCAACG159600.041.7439233
CAACGCA160850.041.3825455
TATCAAC166550.040.29352
ACGCAGA176200.037.9799847
CGCAGAG180500.037.0751958
GCAGAGT195600.034.3042879
GTACATG173050.032.1069641
ACATGGG172900.031.5143723
TACATGG176650.031.4223382
CATGGGG102400.031.020554
TATCACG1802.2027962E-829.7468172
ATGGGGG53600.028.8547125
AGAGTAC186750.028.46047210-11
GAGTACT93200.026.90232712-13
CAGAGTA192000.026.3655310-11
AGTACTT98900.025.622512-13
Produced by FastQC (version 0.11.5)