FastQCFastQC Report
Fri 10 Feb 2017
ERR1147976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604697
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161940.6217229873570708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94600.3631900370753297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77830.29880634868470307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114450.052.704091
GGTATCA63250.051.5906141
AACGCAG141750.042.5079276
TCAACGC150700.039.7860534
CAACGCA152900.039.33035
ATCAACG152350.039.3161133
TATCAAC160200.037.69242
GTATACG1304.177855E-836.6435281
ACGCAGA165250.036.462937
CGCAGAG169150.035.551898
GCAGAGT181250.033.3097659
GTACATG160500.031.4238241
TACATGG164750.030.4044342
ACATGGG163800.030.2129383
CATGGGG91300.028.665894
AGAGTAC173000.028.09050410-11
ATGGGGG46650.028.0514035
GAGTACT85450.026.24296212-13
CAGAGTA178200.026.0357710-11
AGTACTT88750.025.66929612-13
Produced by FastQC (version 0.11.5)