FastQCFastQC Report
Fri 10 Feb 2017
ERR1147975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344251
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334981.0016592654080092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180550.539881725384847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160770.4807354471898192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50520.15106521609771514No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43760.13085142233642152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA229450.052.2061651
GGTATCA125450.045.6073271
AACGCAG270500.043.890036
TATCAAC296150.040.4763072
ATCAACG297150.040.053823
TCAACGC299300.039.8654674
CAACGCA304600.039.1718145
ACGCAGA311650.038.1337
CGCAGAG315400.037.6984678
GCAGAGT337400.035.3285069
GTACATG276100.033.8759651
ACATGGG276100.033.111593
TACATGG281700.032.9861562
CATGGGG155400.032.0366484
ATGGGGG77100.031.707365
AGAGTAC328950.029.08464610-11
CATGGGT41200.028.152054
GAGTACT182200.027.5365412-13
TGGGGGG64500.027.4807876
AGTACTT193350.027.02528212-13
Produced by FastQC (version 0.11.5)