FastQCFastQC Report
Fri 10 Feb 2017
ERR1147974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888820
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204650.5262521793243194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113870.29281375841514906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98570.2534702043293338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159550.053.4704741
GGTATCA86600.045.4410361
AACGCAG195850.043.004996
TCAACGC208250.040.558564
ATCAACG208950.040.3372843
CAACGCA211950.039.850535
TATCAAC222850.038.1217582
ACGCAGA225600.037.3075337
CGCAGAG229100.036.8154648
GTACATG224500.034.3414081
GCAGAGT250750.033.8028349
ACATGGG226350.033.531223
TACATGG230050.033.489212
CATGGGG129350.032.143264
ATGGGGG65500.030.3308325
AGAGTAC240050.028.80516810-11
TGGGGGG56250.026.54186
AGTACTT136850.026.51332712-13
GAGTACT126700.025.91442912-13
CAGAGTA247650.025.01497810-11
Produced by FastQC (version 0.11.5)